The lactate-inducible lldR promoter (BBa_K1847008) fused to RBS(SicP)-sicP-sptP (BBa_K3861028). SicP-SptP is the secretion signal and chaperone for secretion of a POI though the SPI-1 T3SS of Salmonella Typhimurium.1 SptP167 is shortened to the first 167 amino acids that contain the secretion signal and the sicP binding domain needed for functional secretion. SicP is the SptP specific chaperone that unfolds the POI secondary structure for SPI-1 T3SS mediated export.2
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 78
Illegal NheI site found at 101
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
1. Lee, S. H. & Galán, J. E. Salmonella type III secretion-associated chaperones confer secretion-pathway specificity. Mol. Microbiol. 51, 483–495 (2004).
2. Stebbins, C. E. & Galán, J. E. Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion. Nature 414, 77–81 (2001).