RNA

Part:BBa_K382019

Designed by: Christina Agapakis   Group: iGEM10_Harvard   (2010-10-26)


ihpRNA construct against Arabidopsis Ger3 allergen

Intron containing hairpin RNAi construct targeting Arabidopsis Germin 3 allergen sequence. Contains PDK intron from pHANNIBAL, assembled using Silver standard 21.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1411
    Illegal NotI site found at 1418
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 895
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1411
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SpeI site found at 1411


[edit]
Categories
//chassis/eukaryote/athaliana
Parameters
None