RNA

Part:BBa_K382015

Designed by: Christina Agapakis   Group: iGEM10_Harvard   (2010-10-26)


ihpRNA construct against Arabidopsis LTP Allergen

Intron containing hairpin RNA construct for RNAi knocktown of Lipid Transfer Protein allergen in Arabidopsis. Intron is PDK intron from pHANNIBAL assembled with 300bp of sense and antisense DNA from Arabidopsis genomic DNA assembled with Silver standard 21

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 260
    Illegal BsaI.rc site found at 1109


[edit]
Categories
//chassis/eukaryote/athaliana
Parameters
None