Plasmid

Part:BBa_K382000

Designed by: Christina Agapakis   Group: iGEM10_Harvard   (2010-10-26)

pORE Open Series Vector with BioBrick MCS (Basta resistance)

Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has no promoter or reporter. Conforms to Silver BioBrick Assembly Standard 21

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4759
    Illegal XbaI site found at 4774
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4759
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794
    Illegal NotI site found at 4765
    Illegal NotI site found at 4787
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4759
    Illegal BglII site found at 7193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4759
    Illegal suffix found in sequence at 4780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4759
    Illegal XbaI site found at 4774
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 2772
    Illegal NgoMIV site found at 6642
    Illegal AgeI site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6663
    Illegal EcoRI site found at 4759
    Illegal XbaI site found at 4774
    Illegal SpeI site found at 4780
    Illegal PstI site found at 4794


[edit]
Categories
//chassis/eukaryote/athaliana
//plasmid/chromosomalintegration
Parameters
None