Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 2406
- 21Illegal BglII site found at 1504
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 2020
Illegal AgeI site found at 2697
- 1000Illegal BsaI.rc site found at 2486
This is a composite part for intracellular expression of LOX2. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, LOX2 is expressed and participates in the production from Polyunsaturated fatty acid to hydroperoxides (OOHs) ,then the resulting peroxides immediately react with lycopene to promote the degradation of lycopene.
Usage and Biology
Lipoxygenase (LOX) attaches oxygen to the acyl groups of polyunsaturated fatty acids or glycerides to form corresponding hydroperoxides. Lipoxygenase and peroxidase are involved in the degradation of lycopene in food, which requires the existence of oxygen and activating cofactors at the same time. Because lycopene is a compound of carotenoids, the biological function of β-carotene in carotenoids is attributed to its ability to scavenge free radicals and physical quenching of singlet oxygen. And produce vitamin A (retinol). Although lycopene can not be converted into vitamin A, it has a strong effect of scavenging singlet oxygen, scavenging free radicals and inhibiting lipid peroxidation. Lycopene has the strongest antioxidant effect among carotenoids, especially at twice the rate of β-carotene. In the process of lycopene degradation caused by lipoxygenase, lipoxygenase first catalyzes the oxidation of unsaturated or polyunsaturated fatty acids, and the resulting peroxide reacts with lycopene to promote the degradation of lycopene, in order to prevent the rancidity of oily food.