Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1663
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 2080
- 1000COMPATIBLE WITH RFC
This is a composite part for intracellular expression of Laccase. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, Laccase is expressed and participates in the production from indigo to colorless substance.
Usage and Biology
Copper atom is usually contained in the laccase, which is one of the common characters of laccase and maintains its oxidizing/reducing reaction. As the key element of reaction center, copper atom is critical to the activity and specificity of laccase since it exists in the area that binds substrate and breaks & forms chemical bonds. According to crystal structure of laccase, most have 3 copper binding sites which bind 4 copper atoms. Laccase has a wide variation of substrates, which includes phenol, aniline, carboxylic acid (and their derivatives), bio-pigments, lignans, organic metal compounds and other non-phenol compounds. The optimum temperature of laccase is relatively low, while it has the maximum catalyzing efficient among environment of low pH. These characters give laccase a promising prospect in food industry, paper industry, textile industry and bio-repairing of soil. Coriolus versicolor from Aphyllophorales, Aphyllophorales, Hymenomycetes, Basidiomycota, is one of the ideal strains which is used for laccase production and have excellent degradation ability to lignans. Laccase in plants is involved in various botanic metabolism pathways, such as synthesis of lignans, synthesis and degradation of pigments and elongation of roots.