Pynr071c-α factor-LOX2-AOX1 Terminator
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 2679
- 21Illegal BglII site found at 1777
Illegal XhoI site found at 1438
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 2293
Illegal AgeI site found at 2970
- 1000Illegal BsaI.rc site found at 2759
This is a composite part for extracellular expression of Lipoxygenase2. LOX2 is expressed and participates in the production from Polyunsaturated fatty acid to hydroperoxides (OOHs) ,then the resulting peroxides immediately react with lycopene to promote the degradation of lycopene. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, the signal peptide, α-factor , is used to express LOX2 outside of the cell.
Usage and Biology
Lipoxygenase (LOX) attaches oxygen to the acyl groups of polyunsaturated fatty acids or glycerides to form corresponding hydroperoxides. Lipoxygenase and peroxidase are involved in the degradation of lycopene in food, which requires the existence of oxygen and activating cofactors at the same time. Because lycopene is a compound of carotenoids, the biological function of β-carotene in carotenoids is attributed to its ability to scavenge free radicals and physical quenching of singlet oxygen. And produce vitamin A (retinol). Although lycopene can not be converted into vitamin A, it has a strong effect of scavenging singlet oxygen, scavenging free radicals and inhibiting lipid peroxidation. Lycopene has the strongest antioxidant effect among carotenoids, especially at twice the rate of β-carotene. In the process of lycopene degradation caused by lipoxygenase, lipoxygenase first catalyzes the oxidation of unsaturated or polyunsaturated fatty acids, and the resulting peroxide reacts with lycopene to promote the degradation of lycopene, in order to prevent the rancidity of oily food.