Pynr071c-α factor-ROX1-AOX1 Terminator
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 2711
- 21Illegal XhoI site found at 1438
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1630
Illegal SapI site found at 1889
This is a composite part for extracellular expression of ROX1. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, the signal peptide, α-factor , is used to express ROX1 outside of the cell.
Usage and Biology
A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it.