Coding

Part:BBa_K3590019

Designed by: Barbora Hrnčířová   Group: iGEM20_GenerationMendelBrno   (2020-10-18)


Dockerin derived from Clostridium thermocellum

Dockerin is a binding domain, typically found in enzymes with cellulolytic activity, as it serves to immobilize them on a protein scaffold (scaffoldin) thanks to its interaction with complementary cohesin domain. The whole protein complex is called the cellulosome. Thanks to the non-covalent interactions between cohesins and dockerins, enzymes are displayed in close proximity with each other as well as close to the surface of the cell. The cohesin-dockerin interaction is species-specific. This dockerin is derived from Clostridium thermocellum and will interact only with the cohesin from the same species (BBa_K3590018).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
Parameters
None