Coding

Part:BBa_K3590016

Designed by: Barbora Hrnčířová   Group: iGEM20_GenerationMendelBrno   (2020-10-18)


Cohesin derived from Bacteroides cellulosolvens

CohBc Cohesin is a binding domain that attaches proteins containing a complementary dockerin domain to a protein scaffold (called scaffoldin) which is usually anchored to the surface of the cell. The whole protein complex is called the cellulosome. Thanks to the non-covalent interactions between cohesins and dockerins, enzymes are immobilized in close proximity with each other as well as close to the surface of the cell. The cohesin-dockerin interaction is species-specific. This cohesin is derived from Bacteroides cellulosolvens and will interact only with the dockerin from the same species (BBa_K3590017).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 323
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 323
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
Parameters
None