Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R Group: iGEM10_Heidelberg (2010-10-22)
The TetR binds to the Tet Operator and by that causes knockdown auf gene expression. (BB-2, RFC12).
Improvement by iGEM ZJUT-China 2021As the improvement of the 10_Heidelberg registered part (BBa_K337028: tet Repressor), we, ZJUT-China 2021 newly registered partBBa_K3885126. through adding a degradation tag ssrA to the 3' end of the tetR (Figure 1), endowing the tetR a novel function—degradation.
Therefore, we gave the tetR a new function and improved this part.
Sequence and Features
- 10Illegal XbaI site found at 10
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 652
- 23Illegal XbaI site found at 10
- 25Illegal XbaI site found at 10
- 1000COMPATIBLE WITH RFC