Difference between revisions of "Part:BBa K3120017"

 
 
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<partinfo>BBa_K3120017 short</partinfo>
 
<partinfo>BBa_K3120017 short</partinfo>
  
This part is the homologous recombination plasmid of N-Cadherin. Using crisper CAS9 technology, homologous recombination can occur between the left arm and right arm of N-Cadherin and the corresponding fragments on the genome, so as to realize the insertion of eGFP and the subsequent Loxp + neomycin resistance gene into the termination codon of N-Cadherin gene of homo sapiens H9(human embryonic stem cell line).
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This part is the homologous recombination plasmid of N-Cadherin. Using crisper CAS9 technology, homologous recombination can occur between the left arm and right arm of N-Cadherin and the corresponding fragments on the genome, so as to realize the insertion of eGFP and the subsequent Loxp + neomycin resistance gene before the termination codon of N-Cadherin gene of homo sapiens H9(human embryonic stem cell line).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:23, 14 August 2019


N-cad LA+Glycine linker+eGFP+loxP+NeoR+loxP+N-cad RA

This part is the homologous recombination plasmid of N-Cadherin. Using crisper CAS9 technology, homologous recombination can occur between the left arm and right arm of N-Cadherin and the corresponding fragments on the genome, so as to realize the insertion of eGFP and the subsequent Loxp + neomycin resistance gene before the termination codon of N-Cadherin gene of homo sapiens H9(human embryonic stem cell line).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2825
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3509
    Illegal SapI.rc site found at 2674
    Illegal SapI.rc site found at 2884