CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system
This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
- Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
- Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.