Composite

Part:BBa_K3054024

Designed by: Dharmik Hitesh Patel   Group: iGEM19_Chalmers-Gothenburg   (2019-10-20)


bphA1 + pTEF1/ pPGK1 + bphA2

The plasmid vector we used had a terminator for yeast in it. Hence we did not add a terminator to the composite. However, adding a terminator to this biobrick should be okay since it has a stop codon in it

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 738
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1624
    Illegal SapI.rc site found at 409


[edit]
Categories
Parameters
None