Regulatory

Part:BBa_K3054000

Designed by: Dharmik Hitesh Patel   Group: iGEM19_Chalmers-Gothenburg   (2019-10-20)


yeast strong constitutive promoter pCCW12

This is the gene promoter CCW12 from Saccharomyces cerevisiae genome. It is a strong constitutive promoter, tested and used in our lab


Reference: Yasaman Dabirian, Xiaowei Li, Yun Chen, Florian David, Jens Nielsen, and Verena Siewers. Expanding the Dynamic Range of a Transcription Factor-Based Biosensor in Saccharomyces cerevisiae. ACS Synthetic Biology 2019 8 (9), 1968-1975. DOI: 10.1021/acssynbio.9b00144


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/constitutive
Parameters
None