Reporter

Part:BBa_K3026008

Designed by: Francois D Rouleau   Group: iGEM19_ULaval   (2019-09-08)


ToeHold_DNA_MATalpha

ToeHold switch targeting DNA sequence of S. cerevisiae strain BY4742 (MATalpha). This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the DNA sequence of the MATalpha cassette. When this ToeHold is in presence of this DNA sequence, the sfGFP will be produced. This system allows for detection of DNA fragments or plasmids containing this cassette. For in vivo expression, use strain BL21 DE3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 163


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Categories
Parameters
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