Reporter

Part:BBa_K3026000

Designed by: Francois D Rouleau   Group: iGEM19_ULaval   (2019-09-08)


ToeHold_DNA_AmpR

ToeHold switch targeting DNA sequence of pBluescript ampicilin resistance cassette. This part is flanked by Gibson homology sites for plasmid pBluescript for insertion in cloning site. This part, when expressed in a cell or media containing T7 polymerase, will produce a ToeHold switch with a recognition sequence in the DNA sequence of the ampicilin resistance cassette. When this ToeHold is in presence of this DNA sequence, the sfGFP will be produced. This system allows for detection of DNA fragments or plasmids containing this cassette. For in vivo expression, use strain BL21 DE3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 168


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Categories
Parameters
None