RBS region for botR gene from C. botulinum
RBS region for the botR gene in C. botulinum
Usage and Biology
This part entry represents the RBS from the alternative sigma factor-encoding gene botR. BotR is a positive regulator of neurotoxin production. In our project we use the botR RBS as part of the whole regulatory region for the expression of botR comprising PbotR and its native 5'-UTR containing the RBS (BBa_K2992014). This part was also used in conjunction with botR lacking its native promoter to allow for polar expression from the pyrDKE operon on the C. sporogenes genome, following genomic integration of the respective botR-expression module .
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.
Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.