RBS

Part:BBa_K2943900

Designed by: Dror Hadas   Group: iGEM19_TAU_Israel   (2019-10-14)


Improved RBS

This sequence is part of a composite part (http://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (http://parts.igem.org/Part:BBa_K608014).
This sequence is based on the RBS (http://parts.igem.org/Part:BBa_B0032) with several mutations inside and includes also base inserts that increase the Shine Dalgarno(SD) sequence length in order to optimize the expression of the mRFP in the composite part.
The mutations to optimize the SD were made according to the anti-SD sequence on the ribosome and the extra bases which were inserted were according to the the most common optimal distance in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None