Device

Part:BBa_K284022

Designed by: Ane Fernanda Beraldi Zeidler, Luige A. Llerena Calderˇn and Marcos Henrique de Moraes   Group: iGEM09_UNICAMP-Brazil   (2009-10-14)

T4 endolysin under the control of T7 promotor

This device contain the T4 endolysin, a lysozyme from enterobacteria phage, under the control of the T7 promoter. Lysozymes (EC 3.2.1.17) are 1,4-╬▓-N-acetylmuramidases, cleaving the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell.

The T7 promoter is a well-known promoter that has already been characterized. As an example, the Imperial team, in 2007, characterize it for temperature sensitivity. At 37┬║C the number of molecules of GFP synthetized was 3.1013 in 120 minutes (PoPS ~ 1,9.109) (http://parts.igem.org/Part:BBa_I719005).

The part characterization can be found at the Experience section.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 322
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/lysis
//cds/lysis
Parameters
protein