T4 endolysin under the control of T7 promotor
This device contain the T4 endolysin, a lysozyme from enterobacteria phage, under the control of the T7 promoter. Lysozymes (EC 18.104.22.168) are 1,4-╬▓-N-acetylmuramidases, cleaving the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell.
The T7 promoter is a well-known promoter that has already been characterized. As an example, the Imperial team, in 2007, characterize it for temperature sensitivity. At 37┬║C the number of molecules of GFP synthetized was 3.1013 in 120 minutes (PoPS ~ 1,9.109) (http://parts.igem.org/Part:BBa_I719005).
The part characterization can be found at the Experience section.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 322
Illegal AgeI site found at 392
- 1000COMPATIBLE WITH RFC