Coding

Part:BBa_K283005

Designed by: Yu bin, Jiang QinQin   Group: iGEM09_HKU-HKBU   (2009-10-13)

lpp_ompA_eGFP_streptavidin


This part is an improved version of BBa_J36846. BBa_J36846 consists of a signal peptide of lipoprotein (lpp), five transmembrane domains of outer membrane protein A (ompA), and streptavidin, wild-type with His6 tag. It's designed to express streptavidin on the cell surface. To test if the protein successfully expressed on the outer membrane of bacteria, you might need to perform immunostainning analysis or cell fractionation and western blot.

It's a time-consuming work to perform above experiments. To simplify the the procedure and directly observe the expressed protein, we inserted a eGFP-coding sequence lacking a stop codon immediately upstream of streptavidin region. The resulting BBa_K283005 has been demonstrated work as expected (visit our wiki for further information).This part does not pose any biological threat.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
    Illegal AgeI site found at 1285
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
//function/reporter/fluorescence
Parameters
None