RBS

Part:BBa_K2765024

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-30)


1000bp Homologous arm+ura(△yno1)

Knock out gene yno1 through homologous recombination


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 668
    Illegal XhoI site found at 535
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 868
    Illegal BsaI site found at 1669
    Illegal BsaI site found at 2699
    Illegal SapI.rc site found at 1516
    Illegal SapI site found at 2304


[edit]
Categories
Parameters
None