Recombinant plasmid was transformed to E. coli DH5α and cultured at 37 °C for 12 h. Then positive bacteria is cultured in LB culture medium adding with 0.1% ampicillin. After bacteria grows to logarithmic phase, adding AHL until final concentration is10-4 ,10-5,10-6 ,10-7,10-8, 10-9 mol/L. Then light absorption is measured. Excitation/Emission wavelength is 485nm/530nm.
Figure 5 (a) Fluorescence intensity at different time induced by different concentrations of AHL.
(b)A three-dimensional map with log10 concentration of ahl,time and fluorescence intensity as the coordinate
When the AHL concentration is in the range of 10-7~10-8 mol/L, the fluorescence insensity was jump. When the AHL concentration is above 10-7 mol/L, the promoter will respond.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 35
Illegal NheI site found at 58
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 734