Coding

Part:BBa_K2737000

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-06)

AfeR


Recombinant plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR.

A responsive protein from Acidithiobacillus ferrooxidans which can bind with AHL. AHL-AfeR complex can bind at aferR box in the promoter region and activate the expression of genes downstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
    Illegal BglII site found at 316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/aferrooxidans
//function/sensor
Parameters
protein