Recombinant plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR.
A responsive protein from Acidithiobacillus ferrooxidans which can bind with AHL. AHL-AfeR complex can bind at aferR box in the promoter region and activate the expression of genes downstream.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 18
Illegal BglII site found at 316
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC