Synthetic RBS designed for sfGFP
Usage and Biology
This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the pAimX full promoter (BBa_K2675020) or its derivatives (BBa_K2675023, BBa_K2675024, BBa_K2675025) in the composite parts BBa_K2675050, BBa_K2675053, BBa_K2675054, BBa_K2675055, BBa_K2675057, BBa_K2675060, BBa_K2675063, BBa_K2675064 and BBa_K2675065.
Using the Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are: Translation Initiation Rate (au) : 202865,54 dG_total (kcal/mol) : -11,34 dG_mRNA_rRNA (kcal/mol) : -21,58 dG_spacing (kcal/mol) : 0 dG_standby (kcal/mol) : 0 dG_start (kcal/mol) : -2,76 dG_mRNA (kcal/mol) : -13,78 Accuracy (warnings) : OK
 Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.
 Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC