Coding

Part:BBa_K2660009

Designed by: Danielle Biscaro Pedrolli, Victor Nunes de Jesus   Group: iGEM18_Unesp_Brazil   (2018-10-08)


pMag - Light-activated protein


This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools.The positive Magnet (pMag) when irradiated by a blue light change through electrostatic interactions.



This part was engineered to be a building block of the standard BioBrick framework, and can be used with nMag (BBa_K2660008) to join different domains of a protein of interest when irradiated by blue light like N-cas9 (BBa_K2660006) and C-cas9 (BBa_K2660007) domains.


References
Kawano, F. et al. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat. Commun. 6:6256 doi: 10.1038/ncomms7256 (2015)

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//collections/probiotics/biocontainment
Parameters
None