Coding

Part:BBa_K2660006

Designed by: Victor Nunes de Jesus, Danielle Biscaro Pedrolli   Group: iGEM18_Unesp_Brazil   (2018-10-08)


N-Cas9

Coding sequence for the N-terminal section of split Cas9 nuclease.


This BioBrick was engineered to be the N-terminal domain of cas9 (BBa_K2457001) coding sequence from Streptococcus pyogenes. It was designed to be an inactive domain linked with proteins like pMag (BBa_K2660009) to control to control the kinetics of activation by blue light and induce targeted genome sequence modifications (to kill or remove the recombinant DNA). This activity can be switched off simply by extinguishing the light.



The nuclease activity requires the use together with nMag(BBa_K2660008) linked with the c-cas9 domain (BBa_K2660007)


references

Nihongaki, Y et al. Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nature Biotechnology volume 33, pages 755–760 doi: 10.1038/nbt.3245 (2015)


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
//chassis/prokaryote/bsubtilis
//collections/probiotics/biocontainment
//function/crispr/cas9
Parameters
None