Coding

Part:BBa_K2656024

Designed by: Adrián Requena Gutiérrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-22)

mRFP1_LVA Coding Sequence

domestication.
Figure 1. DNA basic parts domestication into GB. Third construction refers to promoter basic pieces.


Part BBa_K2656024 is the Monomeric red fluorescent protein with LVA degradation tag BBa_K1399001 adapted into the Golden Gate assembly method. Thus, it is a basic part compatible with both Biobrick and GoldenBraid 3.0 grammar.

The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.

It can be combined with other compatible parts from our Valencia UPV IGEM 2018 Printeria Collection to assemble transcriptional units in a BsaI one-step reaction with the Golden Gate assembly protocol .


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 556
    Illegal AgeI site found at 668
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
Parameters
None