mRFP1_LVA Coding Sequence
Part BBa_K2656024 is the Monomeric red fluorescent protein with LVA degradation tag BBa_K1399001 adapted into the Golden Gate assembly method. Thus, it is a basic part compatible with both Biobrick and GoldenBraid 3.0 grammar.
The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.
It can be combined with other compatible parts from our Valencia UPV IGEM 2018 Printeria Collection to assemble transcriptional units in a BsaI one-step reaction with the Golden Gate assembly protocol .
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 556
Illegal AgeI site found at 668
- 1000COMPATIBLE WITH RFC