Coding

Part:BBa_K2656023

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-22)

GFPmut3b_LVA Coding Sequence

domestication.
Figure 1. DNA basic parts domestication. Third construction refers to CDS GB domestication.

Part BBa_K2656013 is the GFPmut3b coding sequence with the LVA degradation tag. It is the BBa_K1399004 original sequence adapted to be Golden Gate compatible. Thus, it can be used with Biobrick and Golden Braid assembly methods. The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.

It can be combined with other compatible parts from our Valencia UPV IGEM 2018 Printeria Collection to assemble transcriptional units with a BsaI one-step reaction with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
None