Coding

Part:BBa_K2656021

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-23)

YFP Coding Sequence

domestication.
Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656021 is the Yellow Fluorescent Protein coding sequence BBa_K592101 adapted to the Golden Braid assembly method. Thus, it is a coding sequence compatible with both Biobrick and GoldenBraid 3.0.

This coding sequence can be combined with other Golden Braid 3.0. compatible parts from our Valencia UPV IGEM 2018 Printeria Collection to assemble transcriptional units in a one-step BsaI endonuclease reaction with the Golden Gate assembly protocol . The characterization of this protein (and by extension of all the other part that codify for the YFP) was performed with our transcriptional unit BBa_K2656112. This transcriptional unit was assembled in a Golden Braid alpha1 plasmid including the following parts:

In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. By using this protocol with the parameters of Table 1, Figure 1 was obtained.


Table 1. Parameters used to obtain the spectra
Parameter Value
Number of samples 6
Excitation Wavelength measurement range (nm) [450-550]
Emission wavelenght (nm) 580
Emission Wavelength measurement range (nm) [500-580]
Excitation wavelenght (nm) 470
Gain (G) 50


YFP spectra.
Figure 1. YFP emission and excitation spectra

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/yfp
//function/reporter/fluorescence
Parameters
colorYellow
directionForward
emissionYFP
emit529
excite516
kegg
proteinYFP
swisspro
tagNone