DNA

Part:BBa_K2618000

Designed by: Tim (2018 HKU team)   Group: iGEM18_HK_HCY_LFC   (2018-08-30)


ssDNA for DNA tweezers assembly (Strand 1)

This part is initially transcribed into a non-coding RNA (ncRNA), and then it requires the chassis to possess a HIV reverse transcriptase to reverse transcribe the ncRNA into a single-strand DNA (ssDNA).

The ssDNA interacts with two other ssDNA produced by the parts BBa_K2618001 and BBa K2618002 to form a DNA tweezers. The DNA tweezers can be used to detect a potential biomarker (miRNA 25) of spinocerebellar ataxia type 3.

The ssDNA produced consists of a split G-quadruplex. Upon detection of miRNA 25 by the DNA tweezers, an intact G-quadruplex will be formed. When the G-quadruplex binds with hemin, it will exhibit a peroxidase activity, which can be detected by some colorimetric or fluorescent reagents like ABTS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI site found at 269


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Categories
Parameters
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