ssDNA for DNA tweezers assembly (Strand 1)
This part is initially transcribed into a non-coding RNA (ncRNA), and then it requires the chassis to possess a HIV reverse transcriptase to reverse transcribe the ncRNA into a single-strand DNA (ssDNA).
The ssDNA interacts with two other ssDNA produced by the parts BBa_K2618001 and BBa K2618002 to form a DNA tweezers. The DNA tweezers can be used to detect a potential biomarker (miRNA 25) of spinocerebellar ataxia type 3.
The ssDNA produced consists of a split G-quadruplex. Upon detection of miRNA 25 by the DNA tweezers, an intact G-quadruplex will be formed. When the G-quadruplex binds with hemin, it will exhibit a peroxidase activity, which can be detected by some colorimetric or fluorescent reagents like ABTS.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 101
Illegal SapI site found at 269