Composite

Part:BBa_K2607001

Designed by: Andrea Laurentius   Group: iGEM18_UI_Indonesia   (2018-09-25)


HB-EGF/Tar Receptor (HT) Device

This part is a chimeric between extracellular (binding) domain of Homo sapiens heparin-binding EGF-like growth factor (HB-EGF) receptor and transmembrane/intracellular domain of Escherichia coli Tar chemotaxis receptor.

Usage and Biology

This part can be used to detect diphtheria toxin as it has HB-EGF binding domain. It also can activate chemotaxis pathway in E. coli upon receptor activation as it has Tar transmembrane/intracellular domain.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1434


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