AraC regulated GFP
pBad-GFP regulated by the AraC-ssra deg tag under control of B. burgdorferi PrpoS promoter.
Transformation with Antifreeze Proteins
Biofilm assay plates made from transformed cultures of IaAFP, ZeAFP, GFP, Empty psB1C3 plasmid in EMG competent cells. The goal was to induce the various AFPs in bacteria that are known to form biofilms. This was tested on biofilm assay plates containing a sample of each protein with and without arabinose. The results from the biofilm assay plates show that the samples induced with arabinose produced less biofilm when compared to the same protein sample not induced with arabinose. This is because the arabinose binds to the AraC protein and turns on the operon that triggers the expression of the AFPs.
Image of a protein gel that confirms the expression of the proteins. The protein gel confirms expression of AFPs. The red boxes outline the IaAFP with arabinose, ZeAFP with arabinose and GFP with arabinose all contain bands that show protein expression. The samples of IaAFP, ZeAFP and GFP without arabinose do not show the band that confirms expression of the proteins. The last two wells contained samples of empty psB1C3 plasmid and therefore do not have the bands of protein.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1367
- 21Illegal BamHI site found at 1307
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1552
- 1000COMPATIBLE WITH RFC