Composite

Part:BBa_K2569032

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-10-06)


PampC -mRFP

The mRFP behind constitutive promoter PampC, who is the strongest member in our promoter family, leading to enhanced expression of mRFP. lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP, as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in Pseudomonas fluorescens pf-5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]

Improvement by replacing induced promoter with constitutive promoter

Team SKLMT-China 2018 Improved the part(BBa_J04450)by replacing induced
promoter with constitutive promoter

lacI is present on the genome of many host cells, which suggests that he normal expression of RFP requires the induction of IPTG
and need 18 hours of waiting. We replaced the lac promoter in front of the RFP gene with the strong pf-5 promoter PampC. And hope that RFP,
as a visible marker for the naked eye, can be expressed more pronouncedly and faster in in Pseudomonas fluorescens pf-5.



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