Microglia specific promoter pF4/80
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. The gene is highly expressed in macrophage cells. The combination between pF4/80 and recombinant adeno-associated viruses (rAAV) with capsid variant 6 (AAV6), that has high tropism for monocytes, leads to specifically microglia cells targeting.
Usage and Biology
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene.
We will express Cas9 enzyme with this promoter in order to edit microglia cells genome, for the knockout of IKKβ gene.
Rosario, A. M., Cruz, P. E., Ceballos-Diaz, C., Strickland, M. R., Siemienski, Z., Pardo, M., ... & Golde, T. E. (2016). Microglia-specific targeting by novel capsid-modified AAV6 vectors. Molecular Therapy-Methods & Clinical Development, 3.
Sequence and Features
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