Terminator

Part:BBa_K2486006

Designed by: Cauă Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-22)


Terminator.png

ECK120029600 - Escherichia coli K-12 terminator

  • The spy terminator is the fairly strong palindromic rho-independent transcriptional terminator of the spheroplast protein y ORF. Terminator strength was determined from a simple assay that compares the expression of two fluorescent reporters, one placed before and one after the terminator (Chen et al. 2013).

REFERENCES:

Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. (2013). Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 10(7), 659-664.

https://doulix.com/biomodules/GNO9X1/

Secondary Structure

File:Mfold-K2486006-1.png


Measurement

[edit]
Categories
Parameters
None