Device

Part:BBa_K2427052

Designed by: Mason LaMarche   Group: iGEM17_UIOWA   (2017-10-04)


3-HP responsive regulator and promoter

BBa_K2427052 codes for a MmsR, a Lys-family regulator, which is responsive to 3-hydroxypropionic acid (3-HP). Addition of 3-HP to growth medium is taken up by E. coli, interacts with the MmsR regulator, and permits the accompanying promoter PmmsA to support transcription for downstream coding regions. This allows for inducible expression of proteins downstream from the MmsR-PmmsA system when 3-HP is added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 397
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None