RNA

Part:BBa_K2361004

Designed by: Sebald Verkuijl   Group: iGEM17_Groningen   (2017-10-10)


CRISPR array

This part contains the CRISPR array wihtout any spacers. This array was used to as a backbone to insert spacers into through restriction/ligations using the two BsaI sites. Spacers can be selected based on the desired target to detect.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454
    Illegal BsaI.rc site found at 432


[edit]
Categories
//function/crispr
//function/crispr/cas9
Parameters
None