Guide RNA construct of human Pkp4 (2)
Pkp4 guide RNA construct with a human U6 promoter and human U6 terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 217
- 21Illegal BamHI site found at 183
Illegal XhoI site found at 224
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Usage and Biology
This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the CRISPR dCas13a from Leptotrichia buccalis (LbuC2c2; BBa_K2340000) to form a CRISPR RNA (crRNA).
The sequence that is complementary to Human Pkp4 is ACATTTCCTCACTTCCCTGA. This is one of two Pkp4 guide RNA constructs we have constructed
DNA for this part has not been submitted as a sample to the registry due to time restraints.