Coding
MBP

Part:BBa_K231000

Designed by: Daniel R Tarjan   Group: iGEM09_Virginia   (2009-10-21)

Metal binding peptide

This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well. A similar sequence was reported to successfully increase the ability of E. coli to sequester and survive high levels of heavy metals (Pazirandeh, M., Wells, B., Ryan, R. Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Excherichia coli Expressing a Metal Binding Motif. Appl Environ Microbiol. 64, 4068-4072 (1998).)

Insertion of this gene into K12 derived E. coli strains did not appear to affect growth in a LB. ABP growth curve.png

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds
Parameters
None