Coding

Part:BBa_K2306006

Designed by: Guillermo Serena Ruiz   Group: iGEM17_TUDelft   (2017-10-04)


Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)

This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "CAHS 94205". This is one of the several heat soluble proteins found in the tardigrades responsible for the ability of these microscopic animals to survive the harshest conditions. Upon desiccation, tardigrade proteins form a glass matrix capable of protecting other fragile biological components. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability against dehydration or even enable the possibility of stabilizing material with just a previous drying step. This BioBrick is featured in the composite part BBa_K2306011 combined with a T7 promoter BBa_R0010, a ribosome binding site BBa_k2306014 and a double terminator BBa_B0015 to control its expression. Further information about our project can be found on our results page.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None