Composite

Part:BBa_K2269009

Designed by: José Álvaro Ballesteros González   Group: iGEM17_Valencia_UPV   (2017-09-11)


35s: PhyB: VP16:NLS: Tnos

Unit transcription for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence.


Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3038
    Illegal BglII site found at 1050
    Illegal BglII site found at 1577
    Illegal BamHI site found at 1659
    Illegal XhoI site found at 1610
    Illegal XhoI site found at 1629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3038
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3038
    Illegal AgeI site found at 1070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal EcoRI site found at 3038


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None