Composite

Part:BBa_K2217023

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-24)


YFP for ceRNA circuit characterization

Egypt-AFCM Team Improvement

Egypt-AFCM Team tried to improve YFP gel characterization and function at BBa_K592101 regarding its expression by the lac promoter (BBa_K2217017) as a weak constitutive promoter in one composite part to help enhancing YFP expression to characterize non-coding RNA regulatory activity. Part characterization and usage can be found at BBa_K2217023_Experience.


Part BBa_K2217023 is composed of lac operator (BBa_K2217016) and the lac promoter (BBa_K2217017) as a weak constitutive promoter fused to YFP (BBa_K2217014). Finally, it was terminated by Poly_gh termination (BBa_K2217015).

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection
Parameters
None