Device

Part:BBa_K2205029

Designed by: Lais Takiguchi   Group: iGEM17_Newcastle   (2017-11-01)


Sensynova Framework (Formaldehyde Detector - dCell)

Description

The part BBa_K2205030 was designed as a detector unit of the Sensynova multicellular framework for biosensors development (Figure 1).

Framework generic.jpg

This part is the intermediate part produced in the making of part BBa_K2205030 which was made to test the modularity of the system by replacing the IPTG detector module of the Sensynova platform with the formaldehyde biosensor created by the TMU-Tokyo 2012 team.

This part (BBa_K2205029) consists of a redesign of the original TMU-Tokyo 2012 formaldehyde biosensor part BBa_K749021.

When complete, the system will work through the repression of the part BBa_ K749008 except in the presence of formaldehyde subsequently activating the transcription of connector 1 and therefore triggering the following modules of the platform.

References

iGEM Community. (2012). Team TMU-Tokyo 2012. [online] Available at: http://2012.igem.org/Team:TMU-Tokyo [Accessed 1 Nov. 2017].


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None