Intermediate

Part:BBa_K2205028

Designed by: Lais Takiguchi   Group: iGEM17_Newcastle   (2017-10-27)


Sensynova Framework (PSP3 pag signal tuner Processing Module - pCell) - connector 1B ONLY

Description

The part BBa_K2205025 was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1).

Framework generic.jpg

This part is the intermediate part produced in the making of part BBa_K2205025 which was made to test the modularity of the system by inserting the PSP3 Phage Sensitivity Tuner (BBa_K274381) construct between the two parts of the empty processing module of the Sensynova platform.

In this part (BBa_K2205028), the part BBa_ K274381 is under the control of our connector 1, part BBa_K2205010, however, not yet controlling our connector 2, part BBa_K2205011.

When complete, the system will work through the repression of the part BBa_K274381 except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform.

References

iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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