Sensynova Framework (Arsenic Detector + Connector 1A (complete) - dCell)
The part BBa_K2205022 was designed as a detector unit of the Sensynova multicellular framework for biosensors development (Figure 1) using as a template the partBBa_J33201(design by the Edinburgh team 2006) and introducing the connector 2 CS to allow the entire Sensynova framework to work.
A preliminary qualitative assay was carried out as an initial test for this construct. Co-cultures of Arsenic detector, processor unit (BBa_K2205012) and sfGFP reporter(BBa_K2205015) were inoculated and grown overnight in LB+ Chloramphenicol (12,5ng/ul).
The day after the cultures were diluted at OD600: 0,1 and mixed together to obtain co-cultures with ratio 1:1:13 (detector:processor:reporter). The samples were supplemented with several concentrations of Arsenic (Oppb, 10ppb, 50ppb, 100ppb) to induce the expression of quorum sensing molecules and eventually achieve the reporter visualisation (Figures 3).
This qualitative assay above shows that there is no significant difference among the samples when inoculated with Arsenic in different concentrations and the controls (no Arsenic). Optimisation of the Arsenic detection into the Sensynova framework is required.
Brenner K, Karing D, Weiss R, Arnold F (2007) Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium. Proc Natl Acad Sci USA 104(44): 17300 - 17304
de Mora K, Joshi N, Balint BL, Ward FB, Elfick A, French CE (2011) A pH-based biosensor for detection of arsenic in drinking water. Anal Bioanal Chem 400(4):1031-9 (Epub 2011 Mar 27).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 767
- 1000COMPATIBLE WITH RFC