Device

Part:BBa_K2205020

Designed by: Jessica Birt, Declan Kohl, Bradley Brown   Group: iGEM17_Newcastle   (2017-09-21)


Sensynova Framework (Connector 2B + deGFP (Complete) - rCell)

Description

The part BBa_K2205020 was designed as Reporter unit of the Sensynova multicellular framework for biosensors development(Figure 1).

Framework generic.jpg

For this part, presence of the quorum sensing molecule C4 AHL (Acyl Homoserine Lactone) results in the activate of deGFP transcription.

This part adheres to the the Newcastle iGEM 2017 Sensynova Framework. For additional parts which adhere to this framework, and can therefore be used in conjunction with this part, visit Newcastle 2017 Part collection.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


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Categories
Parameters
None