Device

Part:BBa_K2205015

Designed by: Jessica Birt, Declan Kohl   Group: iGEM17_Newcastle   (2017-09-21)


Sensynova Framework (Connector 2B + sfGFP Reporter (Complete) - rCell)

The part BBa_K2205015 was designed as Reporter unit of the Sensynova multicellular framework for biosensors development(Figure 1).

Framework generic.jpg


In vivo Qualitative Comparison

The device BBa_K2205015 was tested individually after induction with the connector C12-RHL 2 ug/ul as shown in the graph (Figure 2)


Rhl reporter.jpg

Usage and Biology

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
Parameters
None