Device

Part:BBa_K2205010

Designed by: Jessica Birt, Declan Kohl   Group: iGEM17_Newcastle   (2017-09-21)


Sensynova Framework (Connector 1B) - pCell)

Description

LasR coding sequence and the pLas responsive promoter. In the presence of C12 AHL (Acyl Homoserine Lactone), genes downstream of this part are activated by LasR-C12 binding.


This part adheres to the the Newcastle iGEM 2017 Sensynova Framework. For additional parts which adhere to this framework, and can therefore be used in conjunction with this part, visit Newcastle 2017 Part collection.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None