Device

Part:BBa_K2205007

Designed by: Jessica Birt, Declan Kohl   Group: iGEM17_Newcastle   (2017-09-21)


Sensynova Framework (IPTG Detector - dCell)

IPTG responsive system in which constitutively expressed LacI represses the R0010 LacI promoter. In the presence of IPTG, repression of genes downstream of this part is relieved by LacI-IPTG binding.


This part adheres to the the Newcastle iGEM 2017 Sensynova Framework. For additional parts which adhere to this framework, and can therefore be used in conjunction with this part, visit Newcastle 2017 Part collection.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None